Saturday, 11 July 2020

Important steps to reduce ammonia and increase DO


Reducing the feeding rate
It is quite logical to say that controlling the feeding rate reduces the ammonia available in a shrimp pond or system. The feed must be limited to the amount that is consumed. During the summer season, daily feeding rate can be kept up to 100 to 125 pounds per acre. This is an option that offers reasonable results but it cannot be described as a permanent solution to reduce ammonia and increase dissolved oxygen levels.

Using liming agents
Liming agents such as quick lime or hydrated lime can be used to reduce the quantity of ammonia to a certain extent. However, this method is effective in ponds where alkalinity is pretty low. In most fish ponds, alkalinity is moderately high and in such a situation, using liming agents can result in increased levels of PH that is really detrimental to the health of shrimps.

Fertilizing with phosphorous
Majority of the ammonia dispelled by the shrimps is taken by the algae and fertilizing the pond with phosphorous enhances the ammonia uptake.At the same time, dense algae are bloomed under general pond conditions and the algae growth rate is mainly controlled by the light availability instead of the availability of nutrients like phosphorus. Since algae are already growing in a fast manner, fertilizing with phosphorus does not have a major role to play in reducing ammonia concentration.

🦐 Increasing the depth of the pond
When shrimp ponds become deeper, it accommodates more water and the high quantities of water dilute the ammonia to create a better living environment. One of the disadvantages of this solution is that deeper ponds are always highly prone to stratification and the lower layer often contains high amounts of ammonia and low levels of dissolved oxygen.

🦐 Making use of high quality aeration solutions
Since the toxic form of ammoniais a dissolved gas, advanced pond aeration for shrimp farming is the most effective way to eliminate ammonia. Highly advanced aeration solutions speed up the ammonia gas diffusion from pond water to the air for creating an ideal environment for the shrimps. The best way to address high ammonia and low dissolved oxygen is to take action prior to outbreak of a problem. Diffused aeration, or bottom up aeration is the best way to reduce ammonia through aeration. As the bubbles rise up, they diffuse the trapped ammonia into the atmosphere. Also, the higher DO at pond bottom helps the autotrophic “good” bacteria grow faster and creates a natural breakdown of ammonia and other waste. Farmers are able to get a good organic and autotrophic cycle of ammonia reduction and waste removal.

🦐 Other solutions available to reduce ammonia and increase DO include flushing pond with well water, adding an organic carbon source, adding acid, adding bacterial amendments and using ion exchange materials. Although all these methods offer some positive results, making use of the best serration solutions to increase DO levels eliminates all complexities for making the shrimp growth fast and effective 🦐🦐🦐

Saturday, 2 May 2020

Biosynthesis and Characterization of Silver Nanoparticles from Marine Macroscopic Brown Seaweed Colpomenia sinuosa (Mertens ex Roth) Derbes and Solier

Journal of Advanced Chemical Sciences

M. Vishnu Kiran*, S. Murugesan
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant Biology and Biotechnology, Pachaiyappa’s College, University of Madras, Chennai – 600 030, Tamilnadu, India.

M. Vishnu Kiran, S. Murugesan, Biosynthesis and characterization of silver nanoparticles from marine macroscopic brown seaweed Colpomenia sinuosa (Mertens ex Roth) Derbes and
Solier, J. Adv. Chem. Sci. 6(1) (2020) 663–666.

10.30799/jacs.219.20060101

In the present study energy efficient, economically scalable colloidal silver (Ag) nanoparticles were biosynthesized from marine brown seaweed Colpomenia sinuosa by green synthesis method. The marine macroscopic brown seaweed Colpomenia sinuosa was used in the experimental study for the biosynthesis of silver nanoparticle since they are rich in phytochemicals, bioactive compounds and secondary metabolites which has reducing agents that may be environmentally acceptable and eco-friendly. The biosynthesized silver nanoparticles from marine macroscopic brown seaweed were characterized by UV-vis spectroscopy which confirmed the surface plasmon resonance of silver nanoparticles, Fourier transform infrared (FT-IR) spectroscopy to identify the presence of various functional groups in biomolecules responsible for the bio reduction of Ag+ and capping/stabilization of silver nanoparticles. X-ray diffraction (XRD) to observe face center cubic (fcc) and crystalline nature of silver nanoparticles, thermogravimetric analysis (TGA) which revealed the thermal stability and purity of the silver nanoparticles. Particle size distribution and morphology were investigated by scanning electron microscope (SEM) which showed silver nanoparticles in the size range of 54-85 nm. The particle distribution under different nanometers was analyzed using transmission electron microscopy (TEM).

Biosynthesis and Characterization of Silver Nanoparticles from Marine Macroscopic Red Seaweed Halymenia porphyroides Boergesen (Crypton)

M. Vishnu Kiran*, S. Murugesan
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant biology and Biotechnology, Pachaiyappa’s College, University of Madras, Chennai – 600 030, Tamilnadu, India.

https://doi.org/10.30799/jnst.298.20060202

M. Vishnu Kiran, S. Murugesan, Biosynthesis and characterization of silver nanoparticles from marine macroscopic red seaweed Halymenia porphyroides Boergesen (crypton),

J. Nanosci. Tech. 6(2) (2020) 886–890.

In the present study, economically scalable and energy efficient colloidal silver (Ag) nanoparticles were biosynthesized from marine red seaweed Halymenia porphyroides Boergesen (crypton) collected from southeast coast of Tamilnadu, India. The silver nanoparticles were biosynthesized as per green synthesis protocol. The rich presence of phytochemicals, bioactive compounds and secondary metabolites in marine macroscopic red seaweed Halymenia porphyroides Boergesen (crypton) play a major role since they possess reducing and capping agents for the biosynthesis of silver nanoparticles that may be environmentally acceptable and eco-friendly. Therefore, the red seaweed Halymenia porphyroides Boergesen was used in the experimental study for the biosynthesis of silver nanoparticle. The biosynthesized silver nanoparticles from marine macroscopic red seaweed Halymenia porphyroides Boergesen were characterized by UV-vis spectroscopy which confirmed the surface plasmon resonance of silver nanoparticles, Fourier transform infrared (FT-IR) spectroscopy to identify the presence of various functional groups in biomolecules responsible for the bio reduction of Ag+ and capping/stabilization of silver nanoparticles. X-ray diffraction (XRD) to observe face center cubic (fcc) and crystalline nature of silver nanoparticles, thermo gravimetic analysis (TGA) which revealed the thermal stability and purity of the silver nanoparticles. Particle size distribution and morphology were investigated by scanning electron microscope (SEM) which showed silver nanoparticles in the size range of 34.3-80.5 nm. The particle distribution under different nanometers was analyzed using transmission electron microscopy (TEM).

Wednesday, 1 April 2020

Dr Vishnu Kiran Manam speech on basics of viruses

PHYTOCHEMICAL, AMINO ACID, FATTY ACID AND VITAMIN INVESTIGATION OF MARINE SEAWEEDS COLPOMENIA SINUOSA AND HALYMENIA PORPHYROIDES COLLECTED ALONG SOUTHEAST COAST OF TAMILNADU, INDIA

World Journal of Pharmaceutical Research
SJIF Impact Factor 8.084
Volume 9, Issue 4, 1088-1102. Research Article ISSN 2277– 7105

Article Received on
30 Jan. 2020,
Revised on 20 Feb. 2020,
Accepted on 10 March 2020,
DOI: 10.20959/wjpr20204-17091

Corresponding Author
Dr. Vishnu Kiran Manam
Unit of Algal Biotechnology
and Bionanotechnology, PG
and Research Department of
Plant Biology and
Biotechnology,
Pachaiyappa’s College,
Chennai 600 030.

ABSTRACT
Seaweeds are marine macroscopic algae which are considered as the important living marine organisms with high nutritive value and rich bioactive compounds present in the ocean. In the current study the phytochemical, amino acid, fatty acid and vitamin profile analysis of the seaweeds were conducted which were collected along the south east coast of India. The marine brown macro alga Colpomenia sinuosa (Mertens ex Roth) Derbes and Solier as well as the marine red alga
Halymenia poryphyroides Boergesen were used in this study. The relationship between the nutritive components and the variation between phytochemical, amino acid, fatty acid and vitamin profile
were mainly analyzed in the current study. The present analysis revealed that phytochemical contents like alkaloids, triterpenoids, steroids and glycosides were present in higher amounts in marine red
alga Halymenia porphyroides compared to the marine brown alga Colpomenia sinuosa. Similarly the amino acid analysis showed higher percentage in marine red alga Halymenia porphyroides when compared to the marine brown alga Colpomenia sinuosa. The fatty acid profile also revealed higher content in Halymenia porphyroides when compared to Colpomenia sinuosa. On the contrary the vitamin profile analysis revealed higher amounts of vitamins content in Colpomenia sinuosa rather than Halymenia porphyroides. The presence of high phytochemical, amino acid, fatty acid constituents in Halymenia porphyroides makes this seaweed more important when compared Colpomenia sinuosa which were rich in vitamin content and can be used as an important vitamin source for human and animal diet.

Friday, 27 March 2020

Dr Vishnu Kiran Manam Research Gate Profile

Dr Vishnu Kiran Manam LinkedIn Profille

Dr Vishnu Kiran Manam Google Scholar

In vitro Efficacy of Mycorrhizae on Heavy Metal Toxicity in Zea Mays

Int.J.Curr.Microbiol.App.Sci (2014) 3(4): x-xx

ISSN: 2319-7706 Volume 3 Number 4 (2014) pp. x-xx

M.Sathiyanathan1 and M.Vishnu Kiran2*
1PG and Research Department of Botany, Pachaiyappa s College, Chennai, Tamilnadu, India
2Drug Discovery Division, Dr Yellapragada Lifesciences, Chennai 600 077, Tamilnadu, India

Abstract
This study was conducted to know the efficacy of mycorrihizae on heavy metal
toxicity in Zea Mays. Heavy metal toxicity is an increasing problem in agriculture
due to industrialization and urbanization. The application of mycorrhizal for crops
was found to be promising and useful in reducing heavy metal toxicity and
enhancing nutrient uptake. The efficacy of VAM in reducing the heavy metal
toxicity is scantly studied and seldom exploited in heavy metal polluted soil. The
present study aims at understanding the role of VAM in the reduction of heavy
metal toxicity using nitrate reductase system and the role of VAM in the
alleviation, absorption and translocation of heavy metal.

In vitro antioxidant activity of methanolic extract of green alga Valoniopsis pachynema

World Journal of Pharmaceutical Sciences
ISSN (Print): 2321-3310; ISSN (Online): 2321-3086
Published by Atom and Cell Publishers © All Rights Reserved
Available online at: http://www.wjpsonline.org/

K. Kavitha, K. Mahalakshmi and *Vishnu Kiran Manam
Department of Biochemistry, Bhaktavatchalm Memorial College for Women, Korattur, Chennai-80.
Dr Yellapragada Lifesciences, Bio-process Technology Division, Chennai - 600 077. Tamilnadu, India.
Received: 24-08-2015 / Revised: 29-09-2015 / Accepted: 30-09-2015

ABSTRACT
This study was conducted to investigate the in-vitro antioxidant activity of methanolic extract of green alga Valoniopsis pachynema. The free radical scavenging activity of the extracts was measured by superoxide; hydroxyl and nitric oxide radicals and the antioxidant potential were compared with commercial antioxidants, such as Butylated Hydroxy Toluene (BHT) and L- ascorbic acid. The antioxidant activity of experimental algae showed a dose dependent increase when compared to commercial antioxidants. The significant free radical scavenging activity of Valoniopsis pachynema might be attributed to algal contents of carotenoids, free phenols and fatty acids.

In vitro cytotoxic activity of silver nano particle biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines

World Journal of Pharmaceutical Sciences
ISSN (Print): 2321-3310; ISSN (Online): 2321-3086
Published by Atom and Cell Publishers © All Rights Reserved
Available online at: http://www.wjpsonline.org/

Vishnu Kiran M and Murugesan S
Unit of Algal Biotechnology and Bio-Nanotechnology, PG and Research Department of
Botany, Pachaiyappa’s College, Chennai 600 030
Received: 15-08-2014 / Revised: 25-08-2014 / Accepted: 27-08-2014

Abstarct
This study was conducted to investigate the invitro cytotoxic activity of silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides using DLA and EAC cell lines by tryphan blue dye exclusion technique and MTT assay using Mouse L929 cell lines (Lungs fibroblast). The results of the trypan blue dye exclusion assay indicates that the silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides inhibits the growth of DLA and EAC cell lines in a dose dependent manner against the standard drug Curcumin where the silver nano particle biosynthesized from Colpomenia sinuosa showed 61.57 % and silver nano particle biosynthesized from Halymenia poryphyroides showed 89.36 % in DLA cell line similarly the silver nano particle biosynthesized from Colpomenia sinuosa showed 81.96 % and silver nano particle biosynthesized from Halymenia poryphyroides 91.45 % in EAC cell line. The results of the MTT assay indicated the silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides significantly inhibited the proliferation of L929 cells in dose dependent manner where the silver nano particle biosynthesized from Colpomenia sinuosa showed 37.06 % and silver nano particle biosynthesized from Halymenia poryphyroides showed 100 % against the standard drug Curcumin.

Invitro Antioxidant activity of silver nano-particles from Colpomenia sinuosa and Halymenia poryphyroides

World Journal of Pharmaceutical Sciences
ISSN (Print): 2321-3310; ISSN (Online): 2321-3086
Published by Atom and Cell Publishers © All Rights Reserved
Available online at: http://www.wjpsonline.org/

Vishnu Kiran M and Murugesan S
Unit of Algal Biotechnology and Bio-Nanotechnology, PG and Research Department of
Botany, Pachaiyappa’s College, Chennai 600 030
Received: 01-07-2014 / Revised: 15-07-2014 / Accepted: 24-07-2014

Abstract
This study was conducted to investigate the in-vitro antioxidant activities of silver nano particles biosynthesized from Colpomenia sinuosa and Halymenia poryphyroides. The free radical scavenging activity of the extracts was measured by DPPH and ABTS and the antioxidant potential were compared with commercial antioxidants, such as Butylated Hydroxy Toulene (BHT) and L- ascorbic acid. The antioxidant activity of experimental algae showed a dose dependent increase when compared to commercial antioxidants. Spectrophotometric determination of the active compounds revealed that the antioxidant activity might be attributed to algal contents of carotenoids, free phenols and fatty acids.

Biogenic silver nanoparticles by Halymenia poryphyroides and its in vitro anti-diabetic efficacy

Journal of Chemical and Pharmaceutical Research, 2013, 5(12):1001-1008
ISSN : 0975-7384
CODEN(USA) : JCPRC5

Vishnu Kiran M. and Murugesan S.
Unit of Algal Biotechnology and Bio-Nanotechnology, PG and Research Department of Botany,
Pachaiyappa’s College, Chennai, India

ABSTRACT
Diabetes mellitus is a multifunctional disorder characterized by hyperglycemia resulting from increased hepatic glucose production, diminished insulin secretion resulting in impaired insulin action. The intestinal digestive enzymes α-glucosidase and α-amylase plays a key role in carbohydrate digestion, one main antidiabetic approach is to reduce the post prandial glucose level in blood by inhibition of alpha glucosidase and alpha amylase enzymes. Silver nanoparticles were prepared by green synthesis, where silver nitrate was taken as a metal precursor and marine red alga Halymenia poryphyroides as a reducing and capping agent. The formation of silver nanoparticles was characterized by UV–Nano photometer, FT-IR, SEM and XRD. In the present study invitro antidiabetic activity was studied from the biosynthesis of silver nanoparticles from the marine red alga Halymenia poryphyroides as a pre-requisite for the in vivo studies further. The assay results of silver nanoparticles showed dose dependent significantly (P<0.005) increase in percentage inhibitory activity against α–amylase enzyme, at a concentration of 0.2 mg/ml 26.20 ± 0.02% inhibition was seen and at 1.0 mg/ml 91.30 ± 0.02% inhibition was observed, similarly dose dependent significantly (P<0.005) increase in percentage inhibitory activity against α-glucosidase enzyme was also observed where in at lower concentration of 0.2 mg/ml 33.20 ± 0.01% of inhibition and at higher concentration
of 1.0 mg/ml 89.10 ± 0.01% inhibition were recorded.

Biological synthesis of silver nanoparticles from marine alga Colpomenia sinuosa and its in vitro anti-diabetic activity

American Journal of Bio-pharmacology Biochemistry and Life Sciences [AJBBL] [Online ISSN: 2166-126X ]

Vishnu Kiran, M* and Murugesan, S
Unit of Algal Biotechnology and Bio-Nanotechnology, PG and Research Department of Botany, Pachaiyappa’s College, Chennai 600 030. India.

Published: March 01, 2014 Received: November 25, 2013 AJBBL 2014, Volume 03: Issue 01 Page 01-07 Accepted: January 04, 2014.

ABSTRACT
Diabetes is a clinical syndrome characterized by hyperglycemia due to absolute or relative deficiency of insulin. The intestinal digestive enzymes alpha glucosidase and alpha amylase plays a key role in carbohydrate digestion, one main anti-diabetic approach is to reduce the post prandial glucose level in blood by inhibition of α-glucosidase and α-amylase enzymes. The synthesis of silver nanoparticles is an active area of application research in nanotechnology. Biological means by using plants, algae, fungi, bacteria are been employed for the production of low-cost, energy efficient, and non-toxic silver nanoparticles. Silver nanoparticles were prepared from marine alga Colpomenia sinuosa by green synthesis method and the characterization were determined using various techniques like UV – Nano photometer, XRD, FT-IR and SEM. In the present study antidiabetic activity was studied from the biosynthesis of silver nanoparticles from the marine brown alga Colpomenia sinuosa. The assay results of silver nanoparticles showed dose dependent significantly (P<0.005) increase in percentage inhibitory activity against α–amylase and α–glucosidase enzymes.

Free radical scavenging activity of methanolic extract of green alga Valoniopsis pachynema

World Journal of Pharmaceutical Sciences
ISSN (Print): 2321-3310; ISSN (Online): 2321-3086
Published by Atom and Cell Publishers © All Rights Reserved
Available online at: http://www.wjpsonline.org/

K. Kavitha, K. Mahalakshmi and *Vishnu Kiran Manam
Department of Biochemistry, Bhaktavatchalm Memorial College for Women, Korattur, Chennai-80.
Dr Yellapragada Lifesciences, Bio-process Technology Division, Chennai - 600 077. Tamilnadu, India.

ABSTRACT
This study was conducted to investigate the in-vitro antioxidant activity of methanolic extract of green alga Valoniopsis pachynema. The free radical scavenging activity of the extracts was measured by DPPH and ABTS and the antioxidant potential were compared with commercial antioxidants, such as Butylated Hydroxy Toluene (BHT) and L- ascorbic acid. The antioxidant activity of experimental algae showed a dose dependent increase when compared to commercial antioxidants. The significant free radical scavenging activity of Valoniopsis pachynema might be attributed to algal contents of carotenoids, free phenols and fatty acids.

BIOCHEMICAL INVESTIGATION OF MARINE SEAWEEDS COLPOMENIA SINUOSA AND HALYMENIA PORYPHYROIDES COLLECTED ALONG THE SOUTH EAST COAST OF TAMILNADU, INDIA

European Journal of Biomedical and Pharmaceutical sciences

ejbps, 2020, Volume 7, Issue 3, 414-417.

Dr. Vishnu Kiran Manam* and Dr. Murugesan Subbaiah
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant Biology and Biotechnology, Pachaiyappa’s College, Chennai 600 030.

ABSTRACT
In the current study the biochemical evaluation of the seaweeds were conducted which were collected along the south east coast of India. The marine brown macro alga Colpomenia sinuosa (Mertens ex Roth) Derbes and Solier as well as the marine red alga Halymenia poryphyroides Boergesen were used in this study. The relationship between the nutritive components and the variation of the biochemical composition like organic and inorganic constituents were mainly analyzed in the current study. The present analysis revealed that both the seaweeds were rich in protein content whereas more carbohydrate content was observed in marine red alga Halymenia porphyroides compared to the marine brown alga Colpomenia sinuosa. Similarly the analysis of inorganic biochemical constituents revealed that calcium, magnesium, iron and phosphorus were present in higher amount in marine brown alga Colpomenia sinuosa whereas sodium and potassium contents were observed in higher amount in marine red alga Halymenia porphyroides. The presence of high organic and inorganic biochemical constituents in these seaweeds may be of economic importance and ass potential health food for human diets.

Investigation of Biosynthesized Silver Nano Particles Interaction From Halymenia Porphyroides With The E7 Protein Using Bioinformatics Tool

Biomedical Journal of Scientific and Technical Research

DOI: 10.26717/BJSTR.2017.01.000359

Vishnu Kiran M1, Murugesan S1* and Sivamurugan V2
1Division of Algal Biotechnology and Bio-Nanotechnology, Pachaiyappa’s College, India
2PG and Research Department of Chemistry, Pachaiyappa’s College, India
Received: September 02, 2017; Published: September 15, 2017

Silver nano particles have known to possess anticancer properties in the early stages of virus proliferation. In the present study biosynthesis of silver nano particles from marine red alga Halymenia porphyroides was synthesized and characterized. Studies on protein disulphide bonds
were done using Metal Detector Predicts V2.0 software. All Cys and His residues in amino acid sequences were identified. The study revealed that silver nano particles couple with the sulfhydryl groups led to decrease the disulphide bonds and denatures the E7 protein thus eliminating the possibility of the occurrence of the disease

ISOLATION, IDENTIFICATION AND BIOCHEMICAL CHARACTERIZATION OF VIBRIOS SPECIES FROM SHRIMP HATCHERIES NEAR PONDICHERRY COAST, TAMILNADU, INDIA

International Journal of Engineering Applied Sciences and Technology, 2019
Vol. 4, Issue 7, ISSN No. 2455-2143, Pages 125-129
Published Online November 2019 in IJEAST (http://www.ijeast.com)

Dr. Vishnu Kiran Manam
Microbiology Division Department of Microbiology
Dr Yellapragada Lifesciences

 Dr. G. Sumathi
Sri Muthukumaran Institute of Medical Sciences
Chennai, Tamilnadu, India Chennai, Tamilnadu, India.

One of the major fastest growing food sectors globally seems to be the aquaculture industry which ensures a high protein resource to the world due to the exploitation of natural protein resources. One of the major problem in shrimp culture is the disease caused by the bacterial invasions particularly that of vibrios. Some of the important vibrio species that causes the devastation are Vibrio harveyi, Vibrio splendidus, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio vulnificus, Vibrio damsella, Vibrio anguillarum, Vibrio orientalis etc. In the present study the vibrios associated with shrimp hatcheries near Pondicherry coast were isolated, identified and biochemical studies were performed along with the vibrio load from the water samples taken from the larval rearing section, post larval rearing section and nauplli. The isolated and identified vibrios were found out to be Vibrio vulnificus, Vibrio alginolyticus and Vibrio parahaemolyticus based on the biochemical characterization and by selective media plating.

Bio-synthesis of silver nano particles from marine alga Halymenia Poryphyroides and its antibacterial efficacy

M.Vishnu Kiran* and S.Murugesan
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant
biology and Biotechnology, Pachaiyappa s College, Chennai 600 030
*Corresponding author e-mail: dna.vishnu@gmail.com

Int.J.Curr.Microbiol.App.Sci (2014) 3(4): x-xx

Abstract
In this study biosynthesis of colloidal silver (Ag) nano particles from marine alga
Halymenia poryphyroides were studied against Gram-positive and Gram-negative
bacteria. Green synthesis of silver nano particles that have environmentally
acceptable solvent systems and eco-friendly reducing agents is of great importance.
The efficacy was performed using Kirby Bauer Method and the extra cellular
mechanism of silver nano particle creation was characterized by UV-vis
spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy and X-ray
Diffraction (XRD). Particle size distribution and morphology were investigated by
Scanning Electron Microscope which showed silver nano particles in the size range
of 34-80 nm. Fourier transform infrared spectroscopy revealed possible
involvement of reductive groups on the surfaces of silver nano particles. Silver
nano particles showed greater efficacy towards Gram-positive and Gram-negative
bacteria compared to the standard antibiotics

Biosynthesis of silver nanoparticles from marine alga Colpomenia sinuosa and its antibacterial efficacy

M.Vishnu Kiran* and S.Murugesan
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant
biology and Biotechnology, Pachaiyappa s College, Chennai 600 030, Tamilnadu, India
*Corresponding author e-mail: dna.vishnu@gmail.com/smurugesan5@gmail.com

Int.J.Curr.Microbiol.App.Sci (2014) 3(4): x-xx

Abstract
Silver nano particles are known to be good antibiotic agents. In this study
biosynthesis of silver (Ag) nano particles of marine alga Colopomenia sinuosa
were obtained by green synthesis method and their efficacy was studied against
Gram-positive and Gram-negative bacteria. The efficacy was performed using
Kirby Bauer Method and MIC, MBC were also determined. The extra cellular
mechanism of silver nano particle formation was characterized by UV-vis
spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy-ray Diffraction
(XRD), and Scanning Electron Microscopic (SEM) study showed the formation of
silver nano particle in the range of 54-65 nm in size. Silver nanoparticles showed
greater efficacy towards Gram-positive and Gram-negative microorganisms
compared to the standard antibiotics.

An initiative to develop a common repository of algal taxonomic literature for budding algal taxonomists of India

Dear Prof. Baruah and learned Phycologist,
Thank you for your initiative to start the repository of the Algal taxonomic literature of India. It is a nice idea and a much-needed requirement to save algal research in India. 
I will try my best whatever possible to contribute. From Prof. Adhikary I received his cyanobacterial cultures and voucher specimens collected during the AICOPTAX project maintaining it in the Visva-Bharati Culture Collection of Algae. But most of the taxonomic literature is with Dr. Sudipta Kumar Das and Dr. Mrutynjay Jena.  But whatever taxonomic literature of Indian Algae with me I can share with you.

In this initiative, I have a suggestion.  Let's make this literature digital. We can collect the literature to make an Endnote database and put it in a dropbox accessible to all the psychologists of India. If you need any help I can help with this. Since many of the literature are old and not available anywhere it is very much essential to preserve them in the digital platform and if we keep this valuable resource in cloud-like Dropbox it will be easy to accessible to all at the time of need and help students. There will be also new publications from the taxonomic account of algae so we also need to keep windows open for that. So if we make it open in a cloud whoever has a new publication can upload and update it instantly.  Since it is a voluminous work once the platform is ready each one of us can add the literature which we have. 

I have also another suggestion. In this WhatsApp, age why not make a WhatsApp Group of Indian Phycologists but strictly no forwarding messages, Good morning messages etc Just on developments on Indian Algae. So if someone gets a new publication on Algae of India can just WhatsApp in the Group. Thanks to you for collecting the e-mail Id of many of the phycologists of India, we can also keep an Excel sheet in dropbox to include contact details of all phycologists and their specialization, mobile numbers, etc for better coordination and development in this field. 
The legendary Phycologists have a great contribution and we need to carry forward that to the next generation of phycologists. So far we are not well coordinated and because of that loosing also the Algal names our legendary phycologists established in India. For example, the young researches of India use the latest monographs of Komarek for identification of but in that, we don't have many Indian species included. Manual of Prof Desikachary is not available and the student follows Komarek's manual and many Indian species of cyanobacteria now wrongly Identified.
I really appreciate your Initiatives and now in this digital age lets make a digital repository of Algal literature accessible to all.
With my Best Regards

Jnanendra
 --------------------------------------------------------
Dr. Jnanendra Rath 
Raman, NIH Fellow (USA)
Assistant Professor (Stage-III)
Department of Botany
Institute of Science
Visva-Bharati (A Central University)

Monday, 9 March 2020

FACTORS THAT DETERMINE OUR ATTITUDE

Are we born with attitudes or do we develop them as we mature? What are the factors
that form our attitudes?
If you have a negative outlook on life because of your environment, can you change your
attitude? Most of our attitude is shaped during our formative years.
There are primarily three factors that determine our attitude. They are:
1. environment
2. experience
3. education

These are called the triple Es of attitude. Let's evaluate each of the factors individually.
1. Environment
Environment consists of the following:
¨  Home: positive or negative influences
¨  School: peer pressure
¨  Work: supportive or over critical supervisor
¨  Media: television, newspapers, magazines, radio, movies
¨  Cultural background
¨  Religious background
¨  Traditions and beliefs
¨  Social environment
¨  Political environment

All of these environments create a culture. Every place be it a home, organization or a
country has a culture.

Have you noticed that sometimes you go to a store and you find the salesperson polite,
the supervisor, manager and owner polite as well? Yet you go to another shop and you
find everyone rude and discourteous.
You go to a home and you find the kids and parents well-behaved, courteous and
considerate. You go to another home where everyone is fighting like cats and dogs.
In countries where the government and political environment is honest, generally you will
find that the people are honest, law abiding and helpful. And the reverse is true too. In a
corrupt environment, an honest person has a tough time. Whereas in an honest
environment, the corrupt one has a tough time.

In a positive environment, a marginal performer's output goes up. In a negative
environment, a good performer's output goes down.
Culture in any place always goes top down, never bottom up. We need to step back and
look at what kind of environment we have created for ourselves and those around us. It is
tough to expect positive behavior in a negative environment. Where lawlessness
becomes the law, honest citizens become cheats, crooks and thieves.
Isn't it time to evaluate the environment that we are in or we have created for others?

2. Experiences
Our behavior changes according to our experiences with people and events in our life. If
we have a positive experience with a person, our attitude toward him becomes positive
and vice versa.

3. Education
I refer to both formal and informal education, not just academic qualifications. Knowledge
strategically applied translates into wisdom, ensuring success. I talk of education in the
broader sense. It makes the role of the ; educator vital. A teacher affects eternity. The
ripple effect is immeasurable.

We are drowning in information but starving for knowledge and wisdom. Education ought
to teach us not only how to make a living but also how to live.

ACRES OF DIAMONDS

There was a farmer in Africa who was happy and content. He was happy because he
was content. He was content because he was happy. One day a wise man came to him
and told him about the glory of diamonds and the power that goes along with them. The
wise man said, "If you had a diamond the size of your thumb, you could have your own
city. If you had a diamond the size of your fist, you could probably own your own
country." And then he went away. That night the farmer couldn't sleep. He was unhappy
and he was discontent. He was unhappy because he was discontent and discontent
because he was unhappy.
The next morning he made arrangements to sell off his farm, took care of his family and
went in search of diamonds. He looked all over Africa and couldn't find any. He looked all
through Europe and couldn't find any. When he got to Spain, he was emotionally,
physically and financially broke. He got so disheartened that he threw himself into the
Barcelona River and committed suicide.
Back home, the person who had bought his farm was watering the camels at a stream
that ran through the farm. Across the stream, the rays of the morning sun hit a stone and
made it sparkle like a rainbow. He thought it would look good on the mantle piece. He
picked up the stone and put it in the living room. That afternoon the wise man came and
saw the stone sparkling. He asked, "Is Hafiz back?" The new owner said, "No, why do
you ask?" The wise man said, "Because that is a diamond. I recognize one when I see
one." The man said, no, that's just a stone I picked up from the stream. Come, I'll show
you. There are many more." They went and picked some samples and sent them for
analysis. Sure enough, the stones were diamonds. They found that the farm was indeed
covered with acres and acres of diamonds.

Inspirational

There was a man who made a living selling balloons at a fair. He had all colors of
balloons, including red, yellow, blue, and green. Whenever business was slow, he would
release a helium-filled balloon into the air and when the children saw it go up, they all
wanted to buy one. They would come up to him, buy a balloon, and his sales would go up
again. He continued this process all day. One day, he felt someone tugging at his jacket.
He turned around and saw a little boy who asked, "If you release a black balloon, would
that also fly?" Moved by the boy's concern, the man replied with empathy, "Son, it is not
the color of the balloon, it is what is inside that makes it go up."

Students often ask me if they should become entrepreneurs.


My advice is very similar to what Socrates gave about marriage!
“By all means, marry. If you get a good wife, you will be happy. If you get a bad one, you will be a philosopher.”
“By all means, become an entrepreneur . If you do well, you will get rich. And if you do badly, you will learn a lot about the world - and yourself! "