M.Vishnu Kiran* and S.Murugesan
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant
biology and Biotechnology, Pachaiyappa s College, Chennai 600 030
*Corresponding author e-mail: dna.vishnu@gmail.com
Int.J.Curr.Microbiol.App.Sci (2014) 3(4): x-xx
Abstract
In this study biosynthesis of colloidal silver (Ag) nano particles from marine alga
Halymenia poryphyroides were studied against Gram-positive and Gram-negative
bacteria. Green synthesis of silver nano particles that have environmentally
acceptable solvent systems and eco-friendly reducing agents is of great importance.
The efficacy was performed using Kirby Bauer Method and the extra cellular
mechanism of silver nano particle creation was characterized by UV-vis
spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy and X-ray
Diffraction (XRD). Particle size distribution and morphology were investigated by
Scanning Electron Microscope which showed silver nano particles in the size range
of 34-80 nm. Fourier transform infrared spectroscopy revealed possible
involvement of reductive groups on the surfaces of silver nano particles. Silver
nano particles showed greater efficacy towards Gram-positive and Gram-negative
bacteria compared to the standard antibiotics
Unit of Algal Biotechnology and Bionanotechnology, PG and Research Department of Plant
biology and Biotechnology, Pachaiyappa s College, Chennai 600 030
*Corresponding author e-mail: dna.vishnu@gmail.com
Int.J.Curr.Microbiol.App.Sci (2014) 3(4): x-xx
Abstract
In this study biosynthesis of colloidal silver (Ag) nano particles from marine alga
Halymenia poryphyroides were studied against Gram-positive and Gram-negative
bacteria. Green synthesis of silver nano particles that have environmentally
acceptable solvent systems and eco-friendly reducing agents is of great importance.
The efficacy was performed using Kirby Bauer Method and the extra cellular
mechanism of silver nano particle creation was characterized by UV-vis
spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy and X-ray
Diffraction (XRD). Particle size distribution and morphology were investigated by
Scanning Electron Microscope which showed silver nano particles in the size range
of 34-80 nm. Fourier transform infrared spectroscopy revealed possible
involvement of reductive groups on the surfaces of silver nano particles. Silver
nano particles showed greater efficacy towards Gram-positive and Gram-negative
bacteria compared to the standard antibiotics
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